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KMID : 0829320130160010019
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Korean Journal of Clinical Microbiology
2013 Volume.16 No. 1 p.19 ~ p.24
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Comparison Cytomegalovirus Qualitative Assay Using Real-Time PCR and Conventional PCR
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Jeong Se-Ri
Kim Yoon-Jung
Bae Il-Kwon
Jeong Seok-Hoon
Kim Moon-Jung
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Abstract
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Background: Cytomegalovirus (CMV) infection is amajor cause of morbidity and mortality in immunocompromised patients. We compared the abilities of the recently developed Real-Q Cytomegalovirus Kit (Biosewoom Inc., Korea) and the previously used PANA mPCR CMV Detection Kit (Panagene Inc., Korea) to detect CMV.Methods: We analyzed 300 samples (whole blood: 262, urine: 37, CSF: 1) submitted for qualitative CMV PCR testing during October 2011 at Yonsei University College of Medicine Severance Hospital. real-timePCR was performed with a Real-Q Cytomegalovirus Kit and conventional PCR was conducted with a PANA mPCR CMV Detection Kit.Results: The positive rates of both real-time PCR and conventional PCR were 25.3% (76/300), and the kappa coefficient (K) was 0.96 (95% confidence interval(CI), 0.93-1.00). The concordance rate of the Real-Q Cytomegalovirus Kit and the PANA mPCR CMV Detection Kit was 98.7% (296/300), and four out of 300 samples showed discordant results. If theconcordant results of 296 samples and the four results confirmed by direct sequencing were assumed to be true, the sensitivity and specificity of the Real-Q Cytomegalovirus Kit were 97.4% (95% CI,93.8-100.0%) and 99.1% (95% CI, 97.9-100.0%),respectively.Conclusion: The recently developed Real-Q Cytomegalovirus Kit showed excellent sensitivity and specificity, and had a high concordance rate with the previously established PANA mPCR CMV Detection Kit, which uses conventional PCR. Furthermore, real-timePCR could decrease the test time, as the electrophoresis step required for conventional PCR is not required for real-time PCR. (Ann Clin Microbiol 2013;16:19-24)
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KEYWORD
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Cytomegalovirus (CMV), Polymerase chain reaction (PCR), Real-time polymerase chain reaction
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